B12 binding proteins.
نویسندگان
چکیده
منابع مشابه
B12 binding proteins.
A reliable sensitive method for measuring vitamin B12 in the circulation was first achieved at Hammersmith in 1950.' During the subsequent decade the vitamin B12 status was assessed of patients with a wide variety of conditions.2 This culminated in the determination of a unique function for the ileum by Booth and Mollin: that of the absorption of vitamin B123. During the course of these early s...
متن کاملThe vitamin B12 binding power of proteins.
Ternberg and Eakin (1) showed that normal gastric juice or an aqueous extract of hog mucosa when added unheated to vitamin Blz rendered the vitamin non-dializable and unavailable to assay microorganisms, including Lactobacillus leichmannii. They concluded that this principle which combined with vitamin Blz was the intrinsic factor of Castle or an important component thereof. Meyer et al. (2) no...
متن کاملCobalamin (vitamin B12) and B12 binding proteins in hypereosinophilic syndromes and secondary eosinophilia.
Serum cobalamin (vitamin B12) and unsaturated B12 binding capacity (UBBC) have been measured in 24 cases of hypereosinophilia: 16 were cases of hypereosinophilic syndrome (HES) and 8 of secondary eosinophilia. The two groups were similar with respect to absolute eosinophil counts. Serum cobalamin and UBBC were found to be markedly increased in most cases of HES and normal in secondary eosinophi...
متن کاملFractional electrical transport studies of vitamin B12 binding by proteins.
Bird and Hoevet (1) have described a procedure for measuring the vitamin Blz-binding capacity of protein mixtures derived from hog mucosa, based on the non-dialyzability of the protein-bound vitamin. They also observed that lysozyme would bind vitamin B1, as previously reported (3), but that it did not appear to retain the vitamin when dialyzed. However, the diffusibility of lysozyme through ce...
متن کاملIsolation of vitamin B12-binding proteins using affinity chromatography. I. Preparation and properties of vitamin B12-sepharose.
A method of affinity chromatography which is a potent tool for isolation of the trace vitamin Blz binding proteins has been developed. The affinity ligand was prepared by partial acid hydrolysis (0.4 N HCl, 64 hours, room temperature) of the amide groups of the unsubstituted propionamide side chains of the corrin ring of vitamin Blz. The resultant mixture of mono-, di-, and tricarboxylic vitami...
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ژورنال
عنوان ژورنال: Gut
سال: 1990
ISSN: 0017-5749
DOI: 10.1136/gut.31.1.59